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喜報,IF=9.56,西南大學科研團隊揭示蚜蟲翅型分化的分子調控機制

信息來源:金開瑞 作者:genecreate 發布時間:2020-06-01 16:09:09

喜報
       IF=9.56,西南大學植物保護學院王進軍教授團隊近日在《美國科學院院刊》(PNAS)上在線發表題為“The miR-9b microRNA mediates dimorphism and development of wing in aphids”的研究論文,發現小分子RNA介導生物脅迫因子調控蚜蟲翅型分化與翅發育的分子機制,研究結果有利于尋獲新的小分子RNA控制劑靶標,為蚜蟲類害蟲防控提供新的思路。(金開瑞合作技術:IP級多克隆抗體制備)


 
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(信息來源:西南大學植物保護學院和PNAS雜志官網)
 
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合作案例回顧
案例一
Comparative proteomics combined with analyses of transgenic plants reveal ZmREM1.3 mediates maize resistance to southern corn rust. Plant Biotechnology Journal.
合作技術:抗體制備
抗體:ZmREM1.3兔多抗
Western blot analysis of ZmREM1.3 in transgenic maize lines 227, 229, 231 and 233 as well as the corresponding non-transgenic sib lines, with b-actin as the loading control.
案例二
In-depth Proteome of the Hypopharyngeal Glands of Honeybee Workers Reveals Highly Activated Protein and Energy Metabolism in Priming the Secretion of Royal Jelly.
合作技術:抗體制備
抗體:多種兔多抗和鼠單抗
Polyclonal rabbit antibodies against 60S ribosomal proteins RpL28, RpL26, and 40S ribosomal protein S4 (RpS4), and the monoclonal mouse antibody against major royal jelly protein 1 (MRJP1) were developed (Genecreate Biological Engineering, Wuhan, China).
案例三
Divergent molecular evolution in glutathione S-transferase conferring malathionresistance in the oriental fruit fly, Bactrocera dorsalis (Hendel).
合作技術:抗體制備
抗體:制備MR、MS特異性抗體
案例四
CRD1, an Xpo1 domain protein, regulates miRNA accumulation and crown root development in rice, plant journal.
合作技術:抗體制備
抗體:抗CRD 1多克隆抗體
(c) Immunostaining of CRD1-GFP (red fluorescence) in cross-sections of root tips of HJ2 (upper panel) and CRD1-GFP lines (lower panel). (d) Validation of CRD1 antibody (Anti-CRD1) using immunoblot analysis. (e) Subcellular localization of CRD1 in HJ2 analyzed by immunoblot analysis.
案例五
Multiplex immunoassay of chicken cytokines via highly-sensitive chemiluminescent imaging array,Analytica Chimica Acta.
合作技術:抗體制備
抗體:CHIL-4和ChIFN-4的單抗、純化的重組CHIL-4和ChIFN-1抗原
For the first incubation, the surface capture of the cytokines by their respective primary antibodies anti-ChIL-4 and anti-ChIFN-γ, the subsequent CL intensity value increased with increasing incubation time up to a maximum at 30 min (Fig.2a). For the second step, formation of the sandwich via the attachment of the Ab2-AuNP-HRP probe to the exposed Ab1-cytokine-immunocomplex, the maximum CL value was similarly reached with only a 25 min incubation (Fig.2b).



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